23
Dear God, do I know that feel, but I'll do you one better.
I was playing Infamous and I was nearing the end, then I switched to my other file, which was relatively close to the beginning and somewhat close to the middle, because I wanted to play as my evil character. So, my brother came home with a friend and a copy of Infamous 2, saying he wanted to play it, but he wanted to see the end of 1 first. I said okay, went to go load, and forgot that Infamous was that ONE GAME that asks if you save before you load. As a result, I overwrote my near-end file.
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As a result, I had to basically start from the beginning, turn my evil character good, and beat the game as fast as possible just so we could play Infamous 2.
That's not the worst of my save stories, but...d@mn...