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Route 38. It's the most memorable route for me. When I got there while playing SoulSilver, I nearly died from nostalgia. I couldn't remember much about the second gen at all, but I definitely remembered that Miltank farm. I also love the wild Pokemon in that area, too.
Route 1, for some reason I get massive waves of nostalgia when I go there, and it's also just awesome reading Route 1 for some reason. Aside from that, probably Routes 38 and 39 because they have MILTANK.